铁锰改性油茶壳基生物炭对污泥厌氧消化性能及微生物群落结构的影响

doi:10.3969/j.issn.2097-0706.2024.08.006

综合智慧能源 ›› 2024, Vol. 46 ›› Issue (8): 41-49.doi: 10.3969/j.issn.2097-0706.2024.08.006

铁锰改性油茶壳基生物炭对污泥厌氧消化性能及微生物群落结构的影响

罗坤¹^,²(), 朱艺¹^,^*(), 黄兢²^,^*(), 李辉¹^,²()

1.长江大学资源与环境学院，武汉 430100
2.湖南省林业科学院省部共建木本油料资源利用国家重点实验室，长沙 410004

收稿日期:2024-03-06 修回日期:2024-04-01 出版日期:2024-08-25
通讯作者: 朱艺（1982），男，副教授，博士，从事环境污染控制与生态修复方面的研究，zhuyi0731@126.com。
*黄兢（1984），男，副研究员，博士，从事农林剩余物及城市固体废物资源化、湿地及矿区修复等方面的研究，gavinhj@163.com；
作者简介:罗坤（1997），男，硕士生，从事污泥厌氧消化方面的研究，1490899790@qq.com；
李辉（1983），男，研究员，博士，从事生物质资源化、湿地修复、重金属污染土壤修复等方面的研究，lihuiluoyang@163.com。
基金资助:
湖南省杰出青年基金项目(2023JJ10023);长沙市自然科学基金项目(kq2208101)

Impact of iron-manganese modified Camellia oleifera shell-based biochar on the anaerobic digestion performance and microbial community structure of sludge

LUO Kun¹^,²(), ZHU Yi¹^,^*(), HUANG Jing²^,^*(), LI Hui¹^,²()

1. College of Resources and Environment， Yangtze University， Wuhan 430100， China
2. State Key Laboratory of Utilization of Woody Oil Resource， Hunan Academy of Forestry， Changsha 410004， China

Received:2024-03-06 Revised:2024-04-01 Published:2024-08-25
Supported by:
Hunan Outstanding Youth Fund(2023JJ10023);Changsha Natural Science Foundation(kq2208101)

摘要/Abstract

摘要：

水解阶段是污泥厌氧消化的限速阶段，投加外源添加剂生物炭（BC）是打破水解限制与提高甲烷产量的有效手段。以木本油料加工剩余物油茶壳为原料，制备铁锰改性生物炭（Fe-Mn-BC），并通过扫描电镜、傅里叶变换红外光谱仪、X射线光电子能谱、X射线衍射仪等对材料进行表征测试，探讨其对污泥厌氧消化性能、甲烷产量以及微生物群落结构的影响。结果表明：Fe-Mn-BC具有多孔结构，铁锰颗粒以多种氧化物的形式负载在BC表面；添加Fe-Mn-BC能提高甲烷产量，当投加的Fe-Mn-BC总固体质量分数为80 mg/g时，累积产气量最高达301.59 mL/g，较未添加组提升了45.27%。微生物群落分析发现，添加Fe-Mn-BC的组别古菌群落优势菌泉古菌门（Crenarchaeota）、Candidatus_Methanomethylicus和Candidatus_Methanofastidiosum丰度增加，这些菌群能有效促进有机物水解并提高甲烷产量，表明Fe-Mn-BC的加入不仅能富集产甲烷菌等具有功能性的微生物菌群，有效提高污泥厌氧消化效率，也为油茶壳的资源化利用提供了有效途径。

关键词: 厌氧消化, 污泥, 油茶壳, 铁锰改性生物炭, 微生物群落, 甲烷, 水解

Abstract:

Hydrolysis process limits the anaerobic digestion （AD） rate of sludge. Supplementing exogenous biochar （BC） can effectively boost methane production by overcoming the limitation in hydrolysis. The iron-manganese modified biochar （Fe-Mn-BC） derived from the residual shells of woody oil crops， specifically Camellia oleifera shells， is studied. SEM， FTIR， XPS and XRD are employed to characterize the material， and its impacts on sludge AD performance， methane yield and microbial community structure are explored. The study results demonstrate that since Fe-Mn-BC possesses a porous structure， iron and manganese particles can load onto the BC surface in various forms of oxides. The addition of Fe-Mn-BC elevates methane production. When the total solid mass fraction of Fe-Mn-BC reaches 80 mg/g， cumulative gas production peaks at 301.59 mL/g， marking a 45.27% increase compared to that of the control group. Microbial community analysis reveals that Fe-Mn-BC enriches the abundance of archaeal communities， including Crenarchaeota， Candidatus_Methanomethylicus and Candidatus_Methanofastidiosum. These communities play crucial roles in promoting the hydrolysis of organic matters and enhancing the methane production， indicating that Fe-Mn-BC not only enriches functional microbial communities such as methanogenic bacteria， but also effectively improves the efficiency of sludge AD. Furthermore， this method presents a resource utilization solution for Camellia oleifera shells.

Key words: anaerobic digestion, sludge, Camellia oleifera shell, iron-manganese modified biochar, microbial community, methane, hydrolysis

中图分类号:

TK09：X705

罗坤, 朱艺, 黄兢, 李辉. 铁锰改性油茶壳基生物炭对污泥厌氧消化性能及微生物群落结构的影响[J]. 综合智慧能源, 2024, 46(8): 41-49.

LUO Kun, ZHU Yi, HUANG Jing, LI Hui. Impact of iron-manganese modified Camellia oleifera shell-based biochar on the anaerobic digestion performance and microbial community structure of sludge[J]. Integrated Intelligent Energy, 2024, 46(8): 41-49.

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参数	单位	接种污泥	基质污泥
pH值		7.34±0.06	7.42±0.03
TS	g/L	13.62±0.13	79.85±0.46
VS	g/L	5.36±0.04	42.58±0.32
SCOD	mg/L	472.83±26.29	6 183.74±89.15
可溶性蛋白质量浓度	mg/L	43.57±2.49	395.76±2.81
可溶性多糖质量浓度	mg/L	34.59±6.83	402.65±8.22

样品	组成元素的质量分数/%					S_BET/（m²·g^-1）
样品	C	N	O	Fe	Mn	S_BET/（m²·g^-1）
BC	86.43	4.74	8.84	—	—	2.81
Fe-Mn-BC	65.03	—	21.25	7.50	1.92	18.29

试验组	Shannon指数		Simpson指数		Chao1指数		覆盖度
试验组	细菌	古菌	细菌	古菌	细菌	古菌	细菌	古菌
H0	2.558	1.164	0.112	0.361	53.5	8.0	0.999	1.000
H1	2.495	1.234	0.114	0.330	54.5	7.0	0.999	1.000
H2	2.651	1.254	0.100	0.330	51.0	8.0	0.999	1.000
H3	2.681	1.323	0.100	0.313	53.0	8.0	0.999	0.999
H4	2.746	1.462	0.098	0.272	57.0	10.0	0.999	0.999
H5	2.708	1.433	0.099	0.298	56.3	9.0	0.999	0.999

铁锰改性油茶壳基生物炭对污泥厌氧消化性能及微生物群落结构的影响

Impact of iron-manganese modified Camellia oleifera shell-based biochar on the anaerobic digestion performance and microbial community structure of sludge

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